Fig. 5. ROS production in response to IS and hypoxia. RBC obtained from healthy subjects (n=10) were treated with different concentrations of IS (0.01, 0.09, 0.17 mM) for 24h at 37^oC in an incubator with controlled oxygen partial pressure under normoxic (21% O₂) (white bars) or hypoxic (5% O₂) (black bars) conditions. Control RBC (CON-RBC) were incubated with TRIS-Glc-BSA in normoxia and hypoxia. ROS production was analyzed by flow cytometry through DCFH-DA probe fluorescence and results were represented as Mean Fluorescence Intensity (MFI). **p<0.01; ***p<0.001 between normoxia versus hypoxia in the same group. ##p<0.01; ###p<0.001 between CON-RBC vs. IS concentration in the same group (normoxia vs. normoxia; hypoxia vs. hypoxia).